Lumenis Ophthalmology
 
SLT: How & Why It Works
SLT: How & Why It Works

SLT Lowers Pressure With Minimal Damage


Electron microscopy of TM after SLT (note benign nature of SLT therapy)
 
Electron microscopy of TM after ALT

How does SLT achieve lower pressure without damage to the trabecular meshwork?

The underlying mechanism is selective photothermalysis that enables the laser to precisely target intracellular melanin granules to activate individual cells while not disturbing adjacent non-pigmented cells. The activated cells release cytokines that trigger a targeted macrophage response to the trabecular meshwork cells. The macrophages reactivate the meshwork reducing fluid outflow resistance and lowering intraocular pressure.

SLT Uses Frequency Doubled, Q-Switched YAG Laser

For SLT the light energy is provided by a specially designed Q-switched, frequency doubled Nd:YAG laser operating at 532nm green with an output of from 0.3 to 1.5 millijoules (Click here for Lumenis Product info). The target tissue is melanin granules within individual trabecular meshwork cells. Based on the size of the pigment granules, it is necessary to deliver the light energy within 1 microsecond. The Q switched laser pulse width is 3 nanoseconds, well within the required time interval to contain the energy and temperature rise to the pigment granules. Mark Latina, M.D. inventor of SLT did cell culture and animal experiments to determine the specific energy range for selective cell damage. (Click here for Latina paper) Through his investigation parameters were determined which would activate certain pigment containing meshwork cells while sparing immediately adjacent non-pigmented cells. Further human studies showed the pressure lowering effects of Selective Laser Trabeculoplasty without observable damage to the trabecular meshwork cells.

Precise Targeting Not Required for SLT

ALT spot size (left arrow) vs. SLT spot size (right arrow) on TM

Since Selective Laser Trabeculoplasty depends on the laser parameters for targeting the pigmented cells, it is not necessary to have a small spot size or to precisely target a site in the trabecular meshwork as compared to traditional Argon Laser Trabeculoplasty. For SLT a large 400 micron spot is directed into the angle using a specially developed gonio lens to minimize beam distortion and provide good visualization of the angle. Unlike ALT, focus is not critical since the large spot has a greater depth of focus than the 50 micron ALT spot. A red aiming beam the same size as the treatment beam shows the area to be treated. Selective Photothermalysis determines which cells are targeted and activated. During treatment there is normally no observable tissue reaction. Due to variations in pigmentation the laser energy is normally adjusted to a level just below that where a minimally observable effect is achieved.

Selective Targeting of TM Cells

Fluorescent live/dead assay reveals that only melanin-laden trabecular meshwork cells are affected, as seen with red nuclear staining

Selective Laser Trabeculoplasty is an improvement over conventional ALT by eliminating thermal damage of the trabecular meshwork architecture. Using a low-energy, Q-switched, frequency doubled Nd:YAG laser emitting at 532 nm with a pulse duration of 3 nanoseconds, researchers demonstrated isolated destruction of the pigmented TM cells without thermal or collateral damage to the surrounding non-pigmented cells and trabecular collagen beams.

Further, endothelial membrane formation on the TM, usually found in ALT-treated eyes, was not observed after SLT exposure in vivo. Other research using scanning and transmission electron microscopy to compare the acute morphologic changes in the TM of human eye bank eyes after ALT and SLT confirmed these histologic findings. After laser irradiation, ALT produced crater formation, coagulative damage, fibrin deposition and disruption of trabecular beams and endothelial cells. Eyes treated with SLT did not show these findings; the general structure of the TM was preserved. SLT's effect occurred intracellarly, with disruption of the melanin granules. The lack of thermal and structural damage to the TM makes SLT potentially repeatable. *

No Thermal or Collateral Damage

Representation of 400 micron SLT laser beam penetrating through TM

The in vitro and in vivo findings after SLT are due to the much shorter pulse duration (3 nanoseconds) of the treatment compared to the thermal relaxation time of the target chromophore (melanin) in the pigmented TM cells. The melanin granules have a thermal relaxation time of approximately 2 microsecond, while the pulse duration of the SLT is 3 nanoseconds. This means that the pulse duration of SLT is too short for the melanin to transfer the thermal energy; and consequently this spares the surrounding tissues from damage. *

* Source: Review of Ophthalmology, April 2001, David A. Lee, MD & Peter A. Netland, MD, PhD, pgs. 1-2

SLT Utilizes 6000 Times Lower Fluence

When compared to ALT, SLT results in 6000 times less energy density (fluence) being delivered to the tissue. The lower fluence is a result of the larger spot size and the lower energy required. This low fluence is sufficient to activate the desired biologic response at the cellular level without structural damage to the trabecular meshwork.

How SLT Compares With ALT

The following abstracts are from two peer-reviewed articles that compare the SLT and ALT procedures.

Q-switched 532-nm Nd:YAG Laser Trabeculoplasty (Selective Laser Trabeculoplasty) - A Multicenter, Pilot, Clinical Study. Lumenis part number A995.

Comparison of the Morphologic Changes after Selective Laser Trabeculoplasty and Argon Laser Trabeculoplasty in Human Eye Bank Eyes
Lumenis part number 5462.

To order these printed articles in their entirety, please click here and reference the appropriate part numbers.

PB-1004787 Rev A

Lumenis
Selecta II

 

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